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. Author manuscript; available in PMC: 2019 Jun 11.
Published in final edited form as: J Biomol Screen. 2016 Jan 8;21(4):381–390. doi: 10.1177/1087057115624637

Figure 2.

Figure 2.

ADP/ATP exchange assays with human ANT isoforms expressed in isolated yeast mitochondria and Lactococcus lactis. (A) Assay principle (see Results for description). (B) Assay validation with known compounds (10 μM) in isolated yeast mitochondria (left panel) and L. lactis (right panel) using the hANT2 isoform. The ADP/ATP exchange activity was expressed as the percent relative to the DMSO control. Mean ± SD from a single experiment. ANT, adenine nucleotide translocase; TTFA, thenoyltrifluoroacetone; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; MPTP, mitochondrial permeability transition pore