Figure 4.

Detection of PC3-PSCA-Fluc distant metastatic tumors by A11 Mb-IRDye800CW fluorescent imaging.

A, images of multiple distant metastases in SCID mice under the BLI, fluorescence and bright field. In dorsal view, fluorescence imaging identified the paravertebral tumors (arrows) that were detected by bioluminescence (marked by the red dotted lines in the bright field). Fluorescent signal in liver is due to clearance of minibody (arrowhead). In ventral view, the tumors located on the chest and the left thigh were detected by both bioluminescence and fluorescence imaging (arrows). B, ex vivo fluorescent images of metastatic tumors from A. C, H&E staining of the resected fluorescent tissue sample (thigh tumor) confirmed tumor cell infiltration. T, tumor.