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AAV2 expression is detectable 5 months after intravitreal injection. Top: The same retina was imaged for AAV2.AcGFP expression in vivo (left) by Spectralis FA mode, and ex vivo (right) by direct detection with GFP wavelength filter cube. Bottom: Indirect immunofluorescent detection of COMP-Ang1 using a FLAG tag in the AAV2.COMP-Ang1 viral construct (left + inset). FLAG signal is undetectable in untreated retinas (right).
