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. 2019 Jun 6;11:5197–5208. doi: 10.2147/CMAR.S202442

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© 2019 Huang et al.

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Beclin1 overexpression-induced autophagy requires Atg7 and UVRAG in K562 cells and promotes BCR/ABL degradation via an interaction with p62/SQSTM1. (A) The knockdown efficiency of Atg7 or UVRAG by lentiviral vectors in K562 cells was determined via Western blotting. (B) The BCR/ABL and LC3-I/II expression levels in Atg7- and UVRAG-silenced K562 cells were assessed using Western blotting. (C) Fluorescence images showing p62/SQSTM1 (green) and BCR/ABL (red) in the indicated cells (K562-NC, K562-Beclin1, and K562-Beclin1 cells treated with 10 μM CA074 for 24 h). The nuclei were stained with DAPI (blue). The merged images show the colocalization of p62/SQSTM1 and BCR/ABL (yellow). All experiments were repeated at least three times.

Abbreviations: NC, negative control; shRNA, short hairpin RNA.