Figure 4.

Beclin1 overexpression in T315I mutant CML cells induces BCR/ABL downregulation and promotes autophagic cell death. (A) 32Dp210-T315I cells were cultured with different imatinib concentrations for 24 h, and the expression and activation of BCR/ABL protein were assessed via Western blotting. (B) Western blot analysis of BCR/ABL, p-BCR/ABL, Beclin1 and LC3-I/II levels in Beclin1-overexpressing or negative control 32Dp210-T315I cells. (C) CFC assays were performed with BMMCs derived from CML-BC patients with T315I mutant BCR/ABL. The primary cells were infected with LV-Beclin1 or LV-NC. After 14 days of culture in methylcellulose medium, GM-CFUs were observed. All experiments were repeated at least three times.

Abbreviations: BMMCs, bone marrow mononuclear cells; NC, negative control; GM-CFUs, granulocyte-macrophage colony-forming units.