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. 2019 Jun 6;11:5197–5208. doi: 10.2147/CMAR.S202442

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© 2019 Huang et al.

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Beclin1 overexpression downregulates BCR/ABL expression and induces autophagy in CML-LSCs. (A) Representative dot plots showing the purity of the CD34⁺CD38⁻ LSCs collected via FACS from CML primary cells. (B) The CFC assay was performed with CML-LSCs infected with LV-Beclin1 or LV-NC. After 14 days of culture in methylcellulose medium, GM-CFUs were observed and counted (*P<0.05). (C) Western blot analysis of the expression levels of BCR/ABL and other autophagy-related proteins in CML-LSCs with and without Beclin1 overexpression. Three biological replicates were performed for each experiment.

Abbreviations: NC, negative control; FITC, fluorescein isothiocyanate; PE, phycoerythrin; GM-CFUs, granulocyte-macrophage colony-forming units.