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. 2019 Jun 6;14:4145–4155. doi: 10.2147/IJN.S202466

Figure 2.

Figure 2

Fullerol could decrease nitrite production and TNF-α, IL-6 and IL-1β expression in RAW264.7 macrophage cell line.

Notes: Fullerol was not toxic at the doses of up to 3 µM (A, WST-1 assay). Fullerol suppressed LPS-induced nitrite production (B, Griess reagent test) and iNOS gene expression (C, quantitative RT-PCR analysis). It also reversed LPS-stimulated production of TNF-α (D, ELISA assay; E, quantitative RT-PCR analysis) and expression of inflammatory cytokines IL-1β and IL-6 (F, quantitative RT-PCR analysis). LPS: LPS (100 ng/mL) treatment; LPS + FUL: treatment of LPS (100 ng/mL) combined with fullerol (1 µM); LPS + DEX: treatment of LPS (100 ng/mL) combined with dexamethasone (25 µM). For all assays, four repeats were performed in each group (n=4).

Abbreviations: FUL, fullerol; NT, no treatment; iNOS, induced nitric oxide synthase; LPS, lipopolysaccharide; TNF, tumor necrosis factor.