Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (M. musculus) | Pdia1/P4hb | NA | MGI:97464 | |
| Strain, strain background (M. musculus) | Pdia1 fl/fl | Hahm et al., 2013 | ||
| Strain, strain background (M. musculus) | RIP-CreERT | Dor et al., 2004 | ||
| Strain, strain background (M. musculus) | Pdia1 fl/fl;RIP-CreERT | this paper | C57BL/6 mice with Pdia1 floxed alleles were obtained from Dr. J Cho (Univ. of Illinois-Chicago) and crossed with Rat Insulin Promoter (RIP-CreERT) transgenic mice. Congenic Pdia1 gene floxed littermates with or without the CreERT transgene were used for in vivo experiments. | |
| Cell line (M. musculus) | WT MEF | this paper | WT MEF | Freshly prepared primary WT MEFs prior to passage #5 were used for experiments. |
| Adenovirus | human PDI | Wang et al., 2012 | ||
| Adenovirus | human PDImut | Wang et al., 2012 | ||
| Adenovirus | human Proins | this paper | Human proinsulin was cloned into the pAd Easy system. | |
| Antibody | Mouse monoclonal anti-Vinculin | Proteintech | Cat. #: 66305–1-lg | WB (1:2000) |
| Antibody | Rabbit monoclonal anti-GRP94 | Cell signaling Technology | Cat. #: 20292P | WB (1:1000) |
| Antibody | Mouse monoclonal anti-BiP | BD Biosciences | Cat. #: 610979 | WB (1:1000) |
| Antibody | Rabbit polyclonal anti-PDIA1 | Proteintech | Cat. #: 11245–1-AP | WB (1:1000), IHC (1:200) |
| Antibody | Rabbit polyclonal anti-PDIA4 | Proteintech | Cat. #:14712–1-AP | WB (1:1000) |
| Antibody | Rabbit polyclonal anti-PDIA6 |
Proteintech | Cat. #:18233–1-AP | WB (1:1000) |
| Antibody | Mouse monoclonal anti-Proinsulin | HyTest Ltd. | Cat. #: 2PR8 (Mabs: CCI-17) | WB (1:10000), IHC (1:200) |
| Antibody | Guinea pig polyclonal anti-Insulin | this paper | Produced in house WB (1:2000), IHC (1:200) |
|
| Antibody | Mouse monoclonal anti-Glucagon | Abcam | Cat. #: K79bB10 | IHC (1:200) |
| Antibody | IRDye 800CW Goat anti-Mouse IgG (H + L) | Li-Cor | P/N: 926–32210 | WB (1:5000) |
| Antibody | IRDye 680RD Goat anti-Mouse IgG (H + L) | Li-Cor | P/N: 926–68070 | WB (1:5000) |
| Antibody | IRDye 800CW Goat anti-Rabbit IgG (H + L) | Li-Cor | P/N: 926–32211 | WB (1:5000) |
| Antibody | IRDye 680RD Goat anti-Rabbit IgG (H + L) | Li-Cor | P/N: 926–68071 | WB (1:5000) |
| Antibody | IRDye 800CW Donkey anti-Guinea Pig IgG (H + L) | Li-Cor | P/N: 926–32411 | WB (1:5000) |
| Antibody | IRDye 680RD Donkey anti-Guinea Pig IgG (H + L) | Li-Cor | P/N: 926–68077 | WB (1:5000) |
| Antibody | Alexa Fluor 488 goat anti-rabbit IgG | Invitrogen | Cat. #: A-11008 | IHC (1:500) |
| Antibody | Alexa Fluor 488 goat anti-mouse IgG | Invitrogen | Cat. #: A-11001 | IHC (1:500) |
| Antibody | Alexa Fluor 594 goat anti-mouse IgG | Invitrogen | Cat. #: A-11005 | IHC (1:500) |
| Antibody | Alexa Fluor 594 goat anti-guinea pig IgG | Invitrogen | Cat. #: A-11076 | IHC (1:500) |
| Commercial assay or kit | Mercodia Rat/Mouse Proinsulin ELISA | Mercodia | 10-1232-01 | |
| Commercial assay or kit | Mercodia Mouse Insulin ELISA | Mercodia | 10-1247-01 | |
| Commercial assay or kit | CellROX Deep Red reagent | Molecular Probes | C10422 | |
| Chemical compound, drug | DTT | Roche diagnostics | Product #.3117006001 | |
| Chemical compound, drug | Menadione | AdipoGen Life Science | AG-CR1-3631-G001 | |
| Chemical compound, drug | Cycloheximide | Sigma-Aldrich | C7698 | |
| Chemical compound, drug | Brefeldin A | Cell signaling Technology | Cat. #: 9972 | |
| Chemical compound, drug | KSC-34 | Cole et al., 2018 | Obtained from Dr. RL Wiseman (The Scripps Research Institute, La Jolla, CA) |