. Author manuscript; available in PMC: 2019 Jun 11.

Published in final edited form as: Biochemistry. 2019 Jan 31;58(8):1094–1108. doi: 10.1021/acs.biochem.8b01027

Table 2.

Comparison of uracil chemical shifts between GUCU (Figure 9C/D), GUC (Figure 9F), and GUCC (Figure 9E).

Sequence	Residue	H1’	H2’	H3	H5	H6
GUCC	U5	5.608	4.415	ND^a	4.915	7.551
GUCU	U6	5.454	4.027	(10.36)^b	5.12	7.489
GUCU	U18	5.397	4.166	(10.36)^b	5.144	7.533
GUC	U5	5.384	4.143	10.39	5.122	7.426

^a:

Resonance is too broad to be measured

^b:

The assignment of U6 and U18 imino resonances (shown in parenthesis) is based on a small peak at −1°C (Figure S7).