Figure 4.

Analysis of KRAS G12+ CRC patient plasma samples using our droplet PCR assay. a) Representative fluorescent microscope images of droplets following PCR from a Stage IIA cancer patient (i – BF, ii – fluorescent) and a control healthy patient (iii – BF, iv – fluorescent) (Scale bar = 500 μm). Positive droplets were defined as those with a signal-to-noise ratio (SNR) greater than 3.0 and then enumerated in group (b) or as individual patient (c). Y axis = number of detected droplets in 100 μl final volume.