| Genotyping |
|
|
| rec8 T_DNA
|
SAIL_LB3 |
TAGCATCTGAATTTCATAACCAATCTCGATACAC |
|
SAIL_807_B08-RP |
GGGGGAAAAGAGAAAGGTTC |
| rec8 WT allele
|
SAIL_807_B08-LP |
CTCATATTCACGGTGCTCCC |
|
SAIL_807_B08-RP |
GGGGGAAAAGAGAAAGGTTC |
| rec8 WT allelle in REC8:GFP
|
SAIL_807_B08-RP |
GGGGGAAAAGAGAAAGGTTC |
|
TL-gREC8-R |
GAACGGAGAAGGGTAAGGCTCTTGAGTC |
| tam T_DNA
|
SAIL_LB3 |
TAGCATCTGAATTTCATAACCAATCTCGATACAC |
|
TAM_L |
CAGAAATCCTCCACTTGCG |
| tam WT allele
|
TAM_U |
GACTTGATGGATCCACAGC |
|
TAM_L |
CAGAAATCCTCCACTTGCG |
| Cloning of PROREC8:REC8:GFP |
|
| REC8 genome |
AT5G05490-F |
CACCCCAGCCAAGACATTGTGATCTTCAAC |
| REC8 genome |
AT5G05490-R |
TGTGTGATTCAGGGGTAAGAAATATGCG |
| SmaI |
REC8 CterSmaI-F |
GGGTAAGGTTTGATTTCTAAATTA |
| SmaI |
REC8 CterSmaI-R |
GGGCATGTTGGGTCCTCTTGCAAT |
| locus of insertion of gREC8-GFP |
gREC8-GFP_LP |
GAATATTACCTTGCCATAGGCTTG |
| attB1r REC8ter-R |
GGGGACTGCTTTTTTGTACAAACTTGTGTGTGATTCAGGGTAAGAAA |
|
attB4 REC8_2ndI-F |
GGGGACAACTTTGTATAGAAAAGTTAATCAACTCAATTCCCTGTG |
|
attB1r REC8_2ndI-R |
GGGGACTGCTTTTTTGTACAAACTTGGCAAAGAGATAAAACCACGC |
|
REC8 2nd intron-F |
GCCGCCCCCTTCACCGTAATCAACTCAATTCCCTG |
|
REC8 2nd intron-R |
TTCGAATTCCGTTACCTGCAAAGAGATAAAACCAC |
|
Vector for REC8 2I-F |
CTCTTTGCAGGTAACGGAATTCGAAATTTA |
|
Vector for REC8 2I-R |
AGTTGATTACGGTGAAGGGGGCGGCCGCGG |
| Cloning of PROREC8:REC8:GFP/TagRFP:PRORPSA5:TUA5 |
|
|
attB4 TUA5-F |
GGGGACAACTTTGTATAGAAAAGTTTTGATTCGCTATTTGCAGTGCAC |
|
attB1r TUA5-R |
GGGGACTGCTTTTTTGTACAAACTTGTGTGTGATTCAGGGGTAAGAAA |
