Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 May 1;1861(7):1293–1301. doi: 10.1016/j.bbamem.2019.04.007

Fig. 1. Effect of Senp2 on hOAT3 SUMOylation.

Fig. 1.

(a). Top panel: COS-7 cells were cotransfected with hOAT3, HA-SUMO2, Ubc9 together with control vector, Senp2 or inactive mutant of Senp2 for 48h. The cells were then lysed. hOAT3 was immunoprecipitated by anti-Myc antibody (epitope Myc was tagged to hOAT3), followed by immunoblotting with anti-HA antibody to detect SUMOylated hOAT3. Bottom panel: The same immunoblot from Fig. 1a, Top panel was reprobed by anti-Myc antibody. (b). Densitometry plot of results from Fig. 1a as well as from other experiments. The values are mean ± SD (n = 3). *P<0.05. NS: statistically not significant.