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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 May 1;1861(7):1293–1301. doi: 10.1016/j.bbamem.2019.04.007

Fig. 2. Effect of Senp2 on hOAT3 SUMOylation by endogenous SUMO2/3.

Fig. 2.

(a). Top panel: hOAT3 together with control vector, Senp2 or the inactive mutant of Senp2 were co-transfected into COS-7 cells for 48h. The cells were then lysed. hOAT3 was immunoprecipitated by anti-Myc antibody, followed by immunoblotting with anti-SUMO2/3 antibody. Bottom panel: The same immunoblot from Fig. 2a, Top panel was reprobed by anti-Myc antibody. (b). Densitometry plot of results from Fig. 2a as well as from other experiments. The values are mean ± SD (n = 3). *P<0.05. NS: statistically not significant.