Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 May 1;1861(7):1293–1301. doi: 10.1016/j.bbamem.2019.04.007

Fig. 6. (a) Effect of Senp2-specific siRNA on the expression of endogenous Senp2.

Fig. 6.

Top panel: COS-7 cells were co-transfected with hOAT3 and scrambled control siRNA or with hOAT3 and Senp2-specific siRNA. The effectiveness of Senp2-specific siRNA was tested by probing the lysis sample with anti-Senp2 antibody. Bottom panel: The same blot from the top panel was re-probed with anti-actin antibody. β-Actin is a cellular marker protein. (b) Effect of Senp2-specific siRNA on hOAT3 transport activity. COS-7 cells were co-transfected with hOAT3 and scrambled control siRNA or with hOAT3 and Senp2-specific siRNA. Transfected cells were then measured for the uptake of [3H]-estrone sulfate (4-min uptake and 0.3 μM estrone sulfate). The data represent uptake into hOAT3-transfected cells minus uptake into mock cells (parental COS-7 cells). Values are mean ± SD (n = 3). *P < 0.05.