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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 May 1;1861(7):1293–1301. doi: 10.1016/j.bbamem.2019.04.007

Fig. 8. Effect of Senp2-specific siRNA on hOAT3 SUMOylation.

Fig. 8.

(a). Top panel: COS-7 cells were co-transfected with hOAT3, HA-tagged SUMO2, UBC9 and scrambled control siRNA or with hOAT3, HA-SUMO2, UBC9 and Senp2-specific siRNA for 48h. The cells were then lysed. hOAT3 was immunoprecipitated by anti-Myc antibody, followed by immunoblotting (IB) with anti-HA antibody. Bottom panel: The same immunoblot from Fig. 8a, Top panel was reprobed by anti-Myc antibody. (b). Densitometry plot of results from Fig. 8a, as well as from other experiments. The values are mean ± SD (n = 3). *P<0.05.