Table 2.
Validation data for target analytes in water and fish tissue.
| Linear Range | Correlation | Recovery (%, CV) | Precision (%, CV) | Detection Limits (pg) | MDL | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Analyte | (ngmL−1) | Coefficient (r2) | Water | Fish | Water | Fish | LOD | LOQ | Water (ng L−1) | Fish (μg kg−1) |
| ANA | 0.1–20 | 0.999 | 97 (1.2) | 103 (1.3) | 1.9 | 3.4 | 4.0 | 10 | 0.08 | 0.14 |
| CLD | 0.5–100 | 0.998 | 90 (2.6) | 90 (1.8) | 5.0 | 8.2 | 70 | 230 | 0.43 | 0.12 |
| SAX | 0.1–20 | 0.995 | 53 (2.7) | 45 (4.4) | 1.6 | 5.6 | 10 | 40 | 0.22 | 0.04 |
| M-LA | 0.5–100 | 0.998 | 97 (1.8) | 96 (5.7) | 0.8 | 5.7 | 20 | 70 | 0.60 | 0.61 |
| M-LR | 0.1–100 | 0.998 | 94 (2.4) | 97 (5.1) | 0.6 | 5.1 | 40 | 130 | 0.38 | 0.31 |
| M-LY | 0.5–100 | 0.998 | 73 (7.3) | 75 (7.1) | 7.2 | 4.7 | 40 | 140 | 0.83 | 0.35 |
| M-RR | 0.1–100 | 0.999 | 97 (3.0) | 90 (5.9) | 2.4 | 6.3 | 60 | 220 | 0.91 | 0.28 |
| M-YR | 0.5–100 | 0.999 | 93 (4.6) | 77 (4.7) | 3.9 | 1.2 | 80 | 280 | 0.80 | 0.70 |
| NOD | 0.5–100 | 0.996 | 98 (3.1) | 90 (7.9) | 2.9 | 4.7 | 50 | 180 | 0.96 | 0.57 |
Limit of detection (LOD) was defined as LOD = (3σ/b) where σ is the standard deviation of the quantified value from replicate blank samples (n=8), and b is the slope of the calibration for the target analyte. Limit of quantification (LOQ) was defined as LOQ = (10σ/b). Method detection limits (MDL) were defined as MDL = t (n-1,0.99) × SD, where t (n-1,0.99) is the one-sided Student’s t-statistic at the 99% confidence limit for n-1 degrees of freedom, (2.998 for n = 8), and SD is the standard deviation of replicate spiked matrix sample (spiking level ≤ 10 × MDL).