Fig. 1. Comparable antibody reactivity to natural, glycan and common pathogens in ‘resisters’ and LTBI controls.

a, Plasma levels of IgG and IgM reactive to the common natural antigens cardiolipin, phosphatidylserine (PS) and β₂-glycoprotein were profiled across ‘resisters’ (RSTR) (n = 40) and LTBI individuals (n = 39), represented by MFI using a customized Luminex with medians and interquartile ranges depicted for each group. b,c, Plasma IgG and IgM reactivity to 700 glycans in age- and sex-matched ‘resisters’ (n = 5) and LTBI individuals (n = 5) were determined on the NCFGv1 glycan microarray (b) and the CFG mammalian-type glycan microarray CFGv5 (c). Total fluorescence intensities depicted in heatmaps were determined per individual (rows in b and columns in c) and plotted in dot plots as relative fluorescence units (RFU), with medians and interquartile ranges depicted for each group. d, Plasma levels of IgG and IgM reactive to Streptococcus pneumoniae capsular polysaccharides (S. pneu.), a mixture of influenza HA, rubella virus, tetanus toxoid, VZV and CMV pp65 in ‘resisters’ (n = 40) and LTBI individuals (n = 39), were determined using customized multiplex Luminex. AUCs were determined from MFIs generated by three dilutions and plotted for each individual with medians and interquartile ranges depicted for each group. For a–d, statistical significance was calculated using the Mann–Whitney U test, and two-tailed P values are indicated. Dotted lines represent the median level detected in HIV-negative, healthy North American volunteers. e, Principal component analysis using the IgG and IgM data generated in d demonstrates overlapping dot plots of microbial-reactive antibody profiles in ‘resisters’ (n = 40) and LTBI individuals (n = 39). The loadings plot and PLSDA plot are mirror images. Thus, the geographic location of the 12 antibody features on the loadings plot reflects the subject’s group in which a particular feature is enriched.