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. 2019 May 20;25(6):977–987. doi: 10.1038/s41591-019-0441-3

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© The Author(s), under exclusive licence to Springer Nature America, Inc. 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Antibody-dependent cellular phagocytosis of PPD and or ESAT6/CFP10 adsorbed fluorescent FITC beads was measured in THP-1 monocytes. After gating on size, granularity and singlets, the frequency and mean fluorescence intensity of FITC beads was measured. The phagocytic score was calculated as the integrated MFI (percentage frequency × MFI/10,000)⁵⁰. b, Antibody-dependent neutrophil phagocytosis of PPD adsorbed fluorescent FITC beads was measured. Neutrophils were identified by CD66b staining after gating on size, granularity and singlets. The frequency and mean fluorescence intensity of FITC beads was measured, and phagocytic score was calculated as the integrated MFI (percentage frequency × MFI/10,000). c, For antibody-dependent NK-cell activation, cells were first captured after gating on size, granularity and singlets. CD3+ T lymphocytes were gated out and CD16 was used to identify NK cells for which MIP-1β expression, and CD107a and IFN-γ production were measured.