Figure 1. Wilm’s tumor gene (WT1) knockdown inhibits proliferation of B16F10 cells. A: Inhibitory effect of short hairpin RNA against WT1 (shRNAWT1) on B16F10 cells, determined by the MTT assay. B: Assessment of shRNA transfection efficiency and cytotoxicity by fluorescence microscopy. B16F10 cells were transfected with plasmid enhanced green fluorescent protein (pEGFP-N2) as control and shRNA-WT1, and cells were visualized under a visible light and fluorescence light microscopy.
