Figure 4. Expression of Wilm’s tumor gene (Wt1) and B-cell lymphoma 2 (Bcl2) in B16F10 cells. Expression of Wt1 (150 bp), Bcl2 (422 bp), and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) (452 bp) in B16F10 cells treated with short hairpin RNA against WT1 gene (shRNA-WT1) at 50% cytotoxic concentration (CC50) (lane 1); gemcitabine (CC25) (lane 2); gemcitabine (CC25) and shRNA-WT1 (CC50) (lane 3); cisplatin (CC25) with gemcitabine (CC25) (lane 4); cisplatin (CC25) with gemcitabine (CC25) and shRNA-WT1 (CC50) (lane 5); as well as in untreated B16F10 cells (lane 6) and untreated K562 cells (lane 7).