Table 2. Morphological and positive pixel analysis, using three methods of analysis.

Variables	Group	Average	St Dev	Median	Est diff	Confid Int (95%)		P*
Vessel number (HotSpot)	Thrombus	74.3	47.38	52.5	−45.9	−102.9	11.114	0.1019
	Tumour	120.2	46.36	117.5
Vascular area (HotSpot)	Thrombus	52,086.62	32,302	49,759.15	3,308.4	−19,564	26,181	0.751
	Tumour	48,778.21	20,511	46,933.07
PSMA (HotSpot)	Thrombus	0.47	0.15	0.5	−0.025	−0.161	0.1113	0.6888
	Tumour	0.49	0.07	0.5
CD34 (HotSpot)	Thrombus	0.29	0.11	0.28	0.0395	−0.057	0.136	0.3784
	Tumour	0.25	0.07	0.25
PSMA/CD34 (HotSpot)	Thrombus	1.66	1.29	1.22	0.9469	0.1295	1.7644	0.0278*
	Tumour	0.71	0.25	0.69
PSMA (10 HPF Avg)	Thrombus	0.32	0.16	0.33	−0.049	−0.185	0.086	0.4309
	Tumour	0.37	0.08	0.39
CD34 (10 HPF Avg)	Thrombus	0.19	0.11	0.16	0.0236	−0.064	0.111	0.556
	Tumour	0.16	0.06	0.18
PSMA/CD34 (10 HPF Avg)	Thrombus	0.74	0.26	0.71	0.2704	0.0884	0.4523	<0.01*
	Tumour	0.47	0.16	0.46
CD34 (total)	Thrombus	0.3	0.17	0.28	−0.067	−0.202	0.0687	0.2937
	Tumour	0.37	0.07	0.37
PSMA (total)	Thrombus	0.18	0.11	0.18	0.0325	−0.063	0.1276	0.4593
	Tumour	0.15	0.06	0.14
PSMA/CD34 (total)	Thrombus	0.64	0.2	0.64	0.2323	0.0718	0.3927	<0.01*
	Tumour	0.4	0.15	0.39

(I) HotSpot, the most vascular field analyzed; (II) average values obtained from 10 high powered fields (10 HPF Avg); (III) total slide section positive pixel analysis. St Dev, standard deviation; Est Diff, estimated difference; Confid Int, confidence interval. *, P value with <0.05 significance.