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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Ann Biomed Eng. 2019 Apr 8;47(7):1596–1610. doi: 10.1007/s10439-019-02263-8

Figure 3.

Figure 3.

Myotubes exposed to TNF-α exhibit less fusion in 2D culture. The degree to which myoblasts fuse into multinucleated myotubes is another indicator of skeletal muscle maturation. All (A,B) C2C12 and (C,D) hSkM measurements were obtained by quantifying immunofluorescent images. Data are mean±SEM for duplicate wells from 4 donors. 5 randomly chosen images were analyzed per well. A) Average C2C12 myotube diameter. B) Average C2C12 myotube length. C) Average hSkM myotube diameter. D) Average hSkM myotube length.