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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Ann Biomed Eng. 2019 Apr 8;47(7):1596–1610. doi: 10.1007/s10439-019-02263-8

Figure 4.

Figure 4.

TNF-α inhibits myogenesis in engineered human myobundles. A-C: analysis of myotube fusion from MHC immunostaining. (A) average myotube diameter, (B) average nuclei per fiber, and (C) percent of nuclei in MHC positive fibers. (D) mRNA expression analysis of myobundles harvested 5 days after daily TNF-α dosing (0–10,000U/mL) started at the onset of differentiation. Genes of interest were the embryonic (MYH3), perinatal (MYH8), and adult MHC isoforms (MYH1, MYH7). Data are expressed as mean for triplicate wells from 3 donors and 2 biological replicates normalized to TBP.