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. 2019 Jun 11;10:2544. doi: 10.1038/s41467-019-10507-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Structure-specific and sequence-specific recognition of the crRNA repeat region. a and b Details of the interactions between the crRNA repeat region and UrCas13d. c Denaturing gel demonstrating the cleavage of target RNA by wild-type UrCas13d and the mutants in complex with crRNA. d The crRNA repeat region is bound inside the channel formed by NTD, HEPN-1, and HEPN-2 domains of UrCas13d. The nucleotides U(-24)-A(-13) of the crRNA repeat region protrude from UrCas13d. e Denaturing gel demonstrating the cleavage of pre-crRNA or the Δstem mutant (the deletion of the nucleotides U(-24)-A(-21)/U(-16)-A(-13) in pre-crRNA) by wild-type UrCas13d. f Denaturing gel demonstrating the cleavage of target RNA by wild-type UrCas13d in complex with crRNA or the Δstem mutant (the deletion of the nucleotides U(-24)-A(-21)/U(-16)-A(-13) in crRNA). g Denaturing gel demonstrating the cleavage of pre-crRNA or the pre-crRNA mutants by wild-type UrCas13d. h Denaturing gel demonstrating the cleavage of target RNA by wild-type UrCas13d in complex with crRNA or the crRNA mutants