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. 2019 Jun 11;9:8445. doi: 10.1038/s41598-019-44902-z

Figure 2.

Figure 2

Consistency between technical replicates was high at cell input equal to or above 1 K, and there was increased variability at 100 cell input. (a) Heatmap showing Pearson correlation of log2 transformed count values (Blue indicates low correlation and red indicates high correlation). Samples from α-CD3 + B7-1 Fc treatment are shown. (b) PCA plot show global expression pattern for sample in each cell gradient in each platform. Samples from both α-CD3 and α-CD3 + B7-1 Fc treatment are shown. (c) Scatter plots show correlation between the two replicates for each cell gradient in each platform. R2 indicates coefficient of determination. Samples from α-CD3 + B7-1 Fc treatment are shown.