Fig. 7.

Systemic NGF therapy improves recovery from limb ischaemia. (a, b) Line graph (a) and representative laser Doppler images (b) of limb muscle reperfusion. (c–e) Bar graph (c, d) and representative fluorescent microphotographs (e) of capillary and arteriole density in limb muscles of non-diabetic and type 1 diabetic mice treated with Ad.hNGF or Ad.βGal. (e) Capillary endothelial cells are stained with isolectin B4 (green) and arterioles with α-smooth muscle actin (red). Nuclei are stained with DAPI (blue). Scale bar, 50 μm. Bar graphs (c, d) summarise capillary and arteriole density data. In (c) n = 6 per non-diabetic group and type 1 diabetic NGF; n = 7 per type 1 diabetic βGal. In (d) n = 6 per group. Data are expressed as means ± SEM. ^**p < 0.01 vs non-diabetic; ^††p < 0.01 vs type 1 diabetic βGal. ND, non-diabetic; T1DM, type 1 diabetic