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. 2019 Jun 11;10:2543. doi: 10.1038/s41467-019-10485-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Activation of jasmonic acid (JA) signaling reciprocally affects clock activity. Five-day-old seedlings entrained in LD were transferred to LL for 1 day and were treated with JA-isoleucine (JA-Ile). Luminescence was recorded at 1-h intervals for 5 days and analyzed for amplitude, period, and phase with the R package MetaCycle. a–d Expression of CCA1:LUC in Col-0 treated with JA-Ile 25 h (top) or 37 h (bottom) after onset of LL. e–h Expression of CCA1:LUC in coi1-17 treated with JA-Ile 25 h (top) or 37 h (bottom) after onset of LL. i–l Expression of GRP7:LUC in Col-0 treated with JA-Ile 25 h (top) or 37 h (bottom) after onset of LL. a, e, i Luminescence traces. RLU relative luminescence units. The color indicates JA-Ile concentration, black for 0 μM, magenta for 10 μM, and gray for 100 μM. b, f, j Amplitude. c, g, k Period. d, h, l Phase shift. Data represent mean (±SEM) of three independent experiments (n = 8 or 12 for each experiment). Statistical analysis was performed by one-way analysis of variance post hoc Tukey honestly significant difference test. Different letters indicate significant difference among the samples (P < 0.05)