Figure 1.

The switchable pedestal which can record from temporal or parietal cortex in the same rat. (A) The original switchable pedestal with 4 terminals. (B) The customized electrode cables to connect with pedestal. White box shows enlarged illustration of connector pin which attaches to each terminal in the pedestal. (C) The anchor screw was put into the skull of rats at the following coordinates: temporal cortex (AP −4.5 mm, ML −7.5 mm, VD −4.0 mm), parietal cortex (AP −1.0 mm, ML −1.0 mm), frontal sinus for reference (AP 8.0 mm, ML −1.5 mm) and cerebellum for ground (AP −10.0 mm, ML −1.5 mm). The stainless steel wires were attached to the pedestal by soldering at the following position: temporal cortex (red line), parietal cortex (blue line), ground (orange line) and reference (gray line) displayed on the figure. (D) The recording position of ASSR was switchable between temporal and parietal cortex. On the temporal cortex, customized electrode cables were hooked up with EEG-1, ground and reference (red triangle). On the parietal cortex, customized electrode cables were hooked up with EEG-2, ground and reference (blue triangle). (E) Rats were individually placed into a recording box in faraday cage with speakers attached to the cage top, and freely moving during the EEG recording. White box shows enlarged figure of rat with attached electrodes during ASSR recording.