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. 2019 Jun 11;9:8497. doi: 10.1038/s41598-019-43288-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aβ42 induced spectra changes of RB-AuNPs. The normalized Raman spectra of the RB-AuNPs before (violet) and after (green) the addition of Aβ42 peptides (2 μM) show a considerable change in the relative magnitude of the signals from the two major peaks. The orange curve is obtained by digital subtraction. of the (RB-AuNPs) spectrum from the (RB-AuNPs + Aβ42) spectrum. Prior to the subtraction, both spectra were normalized using the standard score method.