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. 2019 Jun 11;10(6):456. doi: 10.1038/s41419-019-1653-7

Fig. 7. Increasing autophagosome accumulation confers GLP-induced cell death and apoptosis in CRC cells.

Fig. 7

a, b HT-29 and HCT116 cells were treated with GLP (5 mg/ml) with or without CQ (10 μM), or Rap (2 μM) for 24 h. The cell viability was then determined by MTT assay. c, d HT-29 and HCT116 cells were treated with GLP (5 mg/ml) with or without CQ (10 μM) (c), or with or without Rap (2 μM) (d) for 24 h. The expression of PARP was determined by western blotting. The relative intensities of cleaved-PARP in each cell line were calculated after normalization against β-Actin. e HT-29 and HCT116 cells were treated with GLP (5 mg/ml), with or without CQ (10 μM) or with or without Rap (2 μM) for 24 h. Apoptosis was analyzed by flow cytometry. Percentage of total apoptotic cells with both early and late apoptosis was calculated and presented. Data are presented as the mean ± SE from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. N.S. No significance