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. 2019 Jun 11;10(6):454. doi: 10.1038/s41419-019-1685-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Wound-healing and b migration assays of Lv-IRF-1 Dox-treated, Lv-IRF-1 Dox-untreated, and Lv-Null Dox-treated MKN45 and AGS cells were performed. c After 48 hours of Dox induction, the expression of six polycistronic miRNAs derived from MIR17HG in the MKN45 and AGS cell lines transfected with Lv-IRF-1 and Lv-Null was analysed by qRT-PCR. d After 48 hours of Dox induction, IRF-1, β-catenin, C-Myc and Axin2 expression in the MKN45 and AGS cell lines transfected with Lv-IRF-1 and Lv-Null was analysed by western blot analysis. e qRT-PCR analysis of the expression of six polycistronic miRNAs derived from MIR17HG in the MKN45 and AGS cell lines after IRF-1 knockdown. f Western blot analysis of IRF-1, β-catenin, C-Myc and Axin2 expression in MKN45 and AGS cell lines after IRF-1 knockdown. All the above experiments were independently performed in triplicate (N = 3). *P < 0.05, as determined by paired Student’s t test. The data a, b, c and e are presented as the means ± SDs