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. 2019 Jun 11;9:8511. doi: 10.1038/s41598-019-44838-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Introduction to the DBTA (dynamic biochemical tissue analysis) (a) DBTA probes (microspheres conjugated with a molecular probe such as a selectin or antibody) are perfused over tissue at defined wall shear stresses using a syringe pump and parallel plate flow chamber. Schematics are not to scale. (b) DBTA probe conjugation with the desired molecular probe (e.g., P-selectin or human IgG) can be verified with flow cytometry. (c) Images from three time points have been combined to show a sample P-selectin DBTA probe rolling on a colon carcinoma tissue.