Figure 6.

P-selectin DBTA probes specifically adhere to consecutive serial sections of colon, lung, ovarian, pancreatic, and stomach cancer tissues. (a) Functional P-selectin ligands are expressed on colon, lung, ovarian, pancreatic, and stomach cancer tissues. DBTA probes were perfused over three serial sections at 0.50 dyne/cm² and 500,000 probes/mL. Specificity of interaction was confirmed using 10 mM EDTA, hIgG DBTA probes, and human P-selectin (hP) DBTA probes perfused over sialidase-treated tissue as negative controls. Data shown are mean adhesion ± SEM of three technical replicates from each of three independent serial sections. * and **P < 0.05 for P-selectin (hP) with respect to the three controls (hP to hP + EDTA, hIgG, and hP + sialidase) and the sialidase-treated tissue with respect to hIgG and hP + EDTA, respectively. See Supplementary Table S1 for P-values and Supplementary Videos S12–S17 for adhesion patterns. (b) Corresponding H&E images on the right are shown in the same order as (a). Core diameter is 2.5 mm.