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. 2019 Jun 11;9:8511. doi: 10.1038/s41598-019-44838-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DBTA detects functional selectin ligands expressed on tissue from multiple solid tumors at both primary and metastatic sites. mE-selectin DBTA probes adhesion to cancerous tissues derived from primary and metastatic sites. DBTA probes were perfused at 500,000 probes/mL and 0.50 dyne/cm². Data shown are mean adhesion per mm² ± SD of three technical replicates and include the deduction of control probe adhesion (viz. mean of P-selectin adhesion minus the greater mean value of either P-selectin + EDTA or IgG control probes).