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. 2019 Jun 11;9:8470. doi: 10.1038/s41598-019-44878-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fgf8b interferes in adipocyte differentiation. (A) The number of mature, lipid-laden adipocytes is reduced at the end of differentiation in a medium containing Fgf8b (125 ng/ml) versus control treated cells (con). Orange colour indicates automatic detection of a lipid droplet by an image analysis algorithm. (B,C) Final lipid droplet number is decreased (B), while size distribution is similar (C) after Fgf8b treatment. (D) Unstimulated (left panel) and isoproterenol-stimulated (iso, right panel) lipolytic rate determined by glycerol release are both decrease after differentiation in the presence of Fgf8b. Bars depict mean values ± SD, n = 3–4. (E) Transcript abundance of brown/brite adipocyte markers UCP1, Cidea and Pgc1a was increased by Fgf8b when normalized to the marker of terminal adipocytes differentiation AP2/Fabp4. Ucp1, uncoupling protein 1; Cidea, cell death-inducing DNA fragmentation factor alpha-like effector A; Pgc1a, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; Cs, citrate synthase; bars are mean values ± SD, n = 3. Stars indicate a significant difference (p < 0.05, Sidak’s multiple comparisons test).