Table 1.
Biochemical assessment of mitochondrial function in epididymal adipose tissue explants from the vicinity of implanted FGF8b release or placebo pellets.
| Placebo | Fgf8b | p | ||
|---|---|---|---|---|
| 1 | adipocyte respiration on G3P (nmol O2/s*g tissue) | 0.84 ± 0.30 | 0.86 ± 0.36 | 0.923 |
| 2 | cytochrome C oxidase activity (nmol O2/s*g tissue) | 3.93 ± 1.64 | 5.24 ± 2.36 | 0.289 |
| 3 | citrate synthase activity (arbitrary unit/g tissue) | 1.12 ± 0.51 | 1.36 ± 0.41 | 0.396 |
| 4 | ratio of 1 to 2 | 0.22 ± 0.04 | 0.17 ± 0.02 | 0.016 |
| 5 | ratio of 1 to 3 | 0.82 ± 0.31 | 0.63 ± 0.20 | 0.244 |
| 6 | ratio of 2 to 3 | 3.74 ± 1.42 | 3.81 ± 1.23 | 0.935 |
1 - Respirometry of isolated, mature adipocytes with glycerol-3-phosphate (G3P) as substrate. 2–Cytochrome C oxidase activity in tissue homogenates. 3 – Citrate synthase activity in tissue homogenates. 4–6 – Ratios between 1–3. None of the parameters assessed was significantly different (Sidak’s multiple comparisons test), n = 6.