TABLE 1.
Strains used to dissect different mechanisms of growth inhibition by acetatea
| Strain | Origin |
|---|---|
| BW25113 | Keio collection |
| BW25113 ΔackA | This study |
| BW25113 Δpta | This study |
| BW25113 Δpta ackA | This study |
| BW25113 Δacs | This study |
| BW25113 Δacs pta | This study |
| BW25113 Δacs pta ackA | This study |
| BW25113 Δacs pta ackA poxB | This study |
| BW25113 Δacs pta ackA::ackAwt | This study |
| BW25113 ΔactP | Keio collection |
| BW25113 ΔsatP | Keio collection |
a
All strains were derived from the wild-type strain of the Keio collection, BW25113 (39). This strain is called the wild type. All deletions developed for this study were constructed without leaving antibiotic resistance cassettes on the chromosome. Notice that Δpta ackA is an abbreviation for the Δpta ΔackA double mutant (a similar notation is used for the other strains). The Δacs pta ackA::ackAwt strain, restoring the original ackA gene, was used as a control.