TABLE 2.
Oligonucleotides used in this study
| Primer namea | Sequence (5′ to 3′)b | Purpose |
|---|---|---|
| acs1P1 | GTTACCGACT CGCATCGGGC AATTGTGGGT TACGATGGCA TCGCGATAGC ATTCCGGGGA TCCGTCGACC | Construction |
| acs2P2 | AACGCTTATG CCACATATTA TTAACATCCT ACAAGGAGAA CAAAAGCATG TGTAGGCTGG AGCTGCTTCG | Construction |
| acs-Right-PCR-verif | AAAACTGCCA ATACCCCT | Verification |
| acs-Left-PCR-verif | TTTTAATTCC CGCTCCCT | Verification |
| ackA-pta-Left-Primer | TGGCTCCCTG ACGTTTTTTT AGCCACGTAT CAATTATAGG TACTTCCATG ATTCCGGGGA TCCGTCGACC | Construction |
| ackA-pta-Right-Primer | GCAGCGCAAA GCTGCGGATG ATGACGAGAT TACTGCTGCT GTGCAGACTG TGTAGGCTGG AGCTGCTTCG | Construction |
| ackA-pta-Left-PCR-verif | CCCTGACGTT TTTTTAGCC | Verification |
| ackA-pta-Right-PCR-verif | CAGCGCAGTT AAGCAAGA | Verification |
| ackA-Right-PCR-verif | TATCCTCTTT CGTTACCGCC | Verification |
| pta-Left-PCR-verif | GGCGGTAACG AAAGAGGA | Verification |
| poxB-seq-Rev | CTCCTTTCTC TCCCATCCC | Verification |
| poxB-seq-Fwd | TAAACGTCGT CCCCAACC | Verification |
| pta-CCDB1 | CTTTCTAGAG AATAGGAACT TCGAACTGCA GGTCGACGGA TCCCCGGAAT TTATATTCCC CAGAACATCA GG | Construction |
| ackA-KN1 | TGGCTCCCTG ACGTTTTTTT AGCCACGTAT CAATTATAGG TACTTCCatg ATAGGAACTT CAAGATCC | Construction |
| Y2-ACKA | CTTTCTAGAG AATAGGAACTT CGAACTGCAG GTCGACGGAT CCCCGGAATc acGGTTTATC CTCTTTCGT | Construction |
| ackA compl verif | CGCAAAATGG CATAGACTCA A | Verification |
a
Note that ackA-Right-PCR-verif and ackA-pta-Left-PCR-verif were used to verify the ackA deletion, pta-Left-PCR-verif and ackA-pta-Right-PCR-verif were used to verify the pta deletion, and ackA-pta-Right-PCR-verif and ackA-compl-verif were used for verifying the ackA complementation.
b
The lowercase sequences in ackA-KN1 and Y2-ACKA represent the start codons of the genes ackA and pta, respectively.