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Detached flagella are frequently seen for ΔfleN bacteria. Live P. aeruginosa WT and ΔfleN bacteria expressing fliC(T394C) were stained with Alexa Fluor 488-maleimide and visualized by fluorescence microscopy. Single polar flagella are clearly visible on most WT cells, and one detached flagellum is indicated by an arrow. ΔfleN cells show multiple polar flagella, and many detached flagellar filaments are observed.
