FIG 5.

TLR2 response to synthetic lipopeptides. (A) HEK-Blue-TLR2/1/6 cells were exposed to 10-fold dilutions of the synthetic lipopeptides Pam₃CSK₄ (Pam3) and PamC(Pam)SK₄ (Pam-Lyso), representing the TA-LP and lyso-LP, respectively, as well as Pam₂CSK₄ (Pam2) and FSL-1, both DA-LP ligands. (B and C) The same lipopeptides were exposed to HEK-Blue cells expressing only TLR2/TLR1 (B) or TLR2/TLR6 (C). (D) HEK-Blue-TLR2/1/6 cells pretreated with 10 μg/ml TLR-neutralizing antibodies were then exposed to two optimized concentrations of Pam₃CSK₄, PamC(Pam)SK₄, and FSL-1. The percent activity when normalized to the water control wells is indicated. The data shown are the means ± standard deviations of the results of three biological replicates.