TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotypea | Reference |
|---|---|---|
| Escherichia coli | ||
| S17-1 | recA pro hsdR RP42Tc::MuKm::Tn7 integrated into the chromosome | |
| BW25113 | K-12 wild type [Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514] | |
| TXM327 | lpp::Cmr | 12 |
| TXM541 | gut::Kanr-rrnB TT-araC-PBAD-lnt lnt::Sptr chiQ::Aprr | 12 |
| KA707 | TXM327 + pLmlit2 | This study |
| KA708 | TXM327 + pEflit2 | This study |
| KA808 | KA708 chiQ::Aprr lnt::Sptr | This study |
| KA811 | KA808 + pKA810 | This study |
| Listeria monocytogenes | ||
| KA694 | CFSAN023459 with plasmid CFSAN023459_02 (courtesy of Dwayne Roberson) | |
| KA738 | KA694 Δlit2 | This study |
| KA834 | KA834 + pPxylLmlit2 | This study |
| KA847 | L2 ATCC 19115 | |
| KA849 | KA847 + pPxylLmlit2 | This study |
| KA1171 | KA738 attB::pPL2 | This study |
| KA1178 | KA738 attB::PpenLmCFSANlit2 | This study |
| KA1179 | KA738 attB::PGram+LmCFSANlit2 | This study |
| Enterococcus faecalis | ||
| TXM465 | ATCC 19433 | |
| KA543 | TXM465 Δlit1 | 12 |
| KA666 | KA543 + pKA635 | 12 |
| KA693 | TX1342 (courtesy of Barbara E. Murray) | |
| GKM744 | KA543 Δlgt | This study |
| GKM760 | TXM465 Δlgt | This study |
| Plasmids | ||
| pKFC | Temperature-sensitive shuttle vector; Cmr | |
| pKFC (ts fix) | Cmr | This study |
| pPxylLmlit2 | pKFC (ts fixed)-PxylLmCFSANlit2; Cmr | This study |
| pKA635 | pMS3535-lit1; Eryr | 12 |
| pKA810 | pCL25-E. coli lppK58A-Strep tag; Trimr | This study |
| plnt | pUC19-E. coli lnt; Carr | 12 |
| pEflit2 | pUC19-E. faecalis TX1342 lit2; Carr | This study |
| pLmLit2 | pUC19-L. monocytogenes CFSAN023459 lit2; Carr | This study |
| pPL2 | Integration into Lm tRNAArg site; Cmr (courtesy of Richard Calendar) | 60 |
| pTXM1170 | pPL2-PpenLmCFSANlit2; Cmr | This study |
| PTXM1169 | pPL2-PGram+LmCFSANlit2; Cmr | This study |
a
Km, kanamycin; Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; Sptr, spectinomycin resistance; Aprr, apramycin resistance; Eryr, erythromycin resistance; Trimr, trimethoprim resistance; Carr, carbenicillin resistance.