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The effect of Lir on cardiomyocytes hypertrophy in vitro. Cardiomyocytes were treated with AngII and/or Lir (25, 50, 100 nM) for 24 h. (A) Cell viability of cardiomyocytes (n = 6). (B) Representative immunofluorescent staining of α-actin (n = 6). (C) Quantitative analysis of CSA (n > 50 cells per group). (D) RT-PCR analysis of the mRNA levels of ANP, BNP, and β-MHC in the indicated groups (n = 6). ∗P < 0.05, compared to the control group; #P < 0.05, compared to the AngII group.
