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. 2019 Jun 5;10:537. doi: 10.3389/fphar.2019.00537

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Copyright © 2019 Li, Shan, Gao, Wang, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of Lir on human cardiomyocytes. AC16 cells were stimulated with Ang II (1 μM) in the presence of Lir (100 nM) and infected with Ad.caAkt. (A) Representative immunofluorescent staining of α-actin (n = 6). (B) Quantitative analysis of CSA (n > 50 cells per group). (C–E) RT-PCR analysis of the mRNA levels of ANP, BNP, and β-MHC in the indicated groups (n = 6). ^∗P < 0.05, compared to the control group; ^#P < 0.05, compared to the AngII group.