Table 1.
Primer sequences used for RT-PCR.
| mRNA | Forward | Reverse |
|---|---|---|
| ANPa | ACCTGCTAGACCACCTGGAG | CCTTGGCTGTTATCTTCGGTACCGG |
| BNP | GAGGTCACTCCTATCCTCTGG | GCCATTTCCTCCGACTTTTCTC |
| α-MHC | GTCCAAGTTCCGCAAGGT | AGGGTCTGCTGGAGAGGTTA |
| β-MHCa | CCGAGTCCCAGGTCAACAA | CTTCACGGGCACCCTTGGA |
| Col1agenIa | AGGCTTCAGTGGTTTGGATG | CACCAACAGCACCATCGTTA |
| Col1agenIIIa | AAGGCTGCAAGATGGATGCT | GTGCTTACGTGGGACAGTCA |
| CTGFa | AGGGCCTCTTCTGCGATTTC | CTTTGGAAGGACTCACCGCT |
| TGFβa | ATCCTGTCCAAACTAAGGCTCG | ACCTCTTTAGCATAGTAGTCCGC |
| GAPDHa | ACTCCACTCACGGCAAATTC | TCTCCATGGTGGTGAAGACA |
| ANPb | AAAGCAAACTGAGGGCTCTGCTCG | TTCGGTACCGGAAGCTGTTGCA |
| BNPb | CAGCAGCTTCTGCATCGTGGAT | TTCCTTAATCTGTCGCCGCTGG |
| β-MHCb | TCTGGACAGCTCCCCATTCT | CAAGGCTAACCTGGAGAAGATG |
| GAPDHb | GACATGCCGCCTGGAGAAAC | AGCCCAGGATGCCCTTTAGT |
| ANPc | CAGCAAGCAGTGGATTGCTCCT | TCTGCGTTGGACACGGCATTGT |
| BNPc | TGGAAACGTCCGGGTTACAGGA | TCCGGTCCATCTTCCTCCCAAA |
| β-MHCc | GGGCAAAGGCAAGGCCAAGAAA | ATGGGTGGAGCGCAAGTTGGTCA |
| GAPDHc | CATCACCATCTTCCAGGAGCGAGA | TGCAGGAGGCATTGCTGATGATCT |
Sequences are listed 5′–3′. aThe PCR used these primers for mice. bThe PCR used these primers for neonatal rat cardiomyocytes. cThe PCR used these primers in the AC cells.