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. 2019 Jun 5;11:135. doi: 10.3389/fnagi.2019.00135

FIGURE 6.

FIGURE 6

Effects of alcohol dehydrogenase 1B (ADH1B) on BACE 1, IDE, and p75NTR expression in SH-SY5Y cells cultured with β-amyloid (Aβ)1-42. After treatment with 10 μM Aβ1-42, cells were divided into four groups: overexpressing ADH1B, overexpressing control, shRNA ADH1B, and shRNA control. (A) Representative expression of Aβ42-related proteins in different groups was determined. Proteins were analyzed using western blotting with antibodies against BACE 1, IDE, and p75NTR. Lane 1, shRNA con group; lane 2, shRNA ADH1B group; lane 3, overexpressing con group; lane 4, overexpressing ADH1B group. (B) Relative quantitation of BACE 1 in the four groups. (C) Relative quantitation of IDE in the four groups. (D) Relative quantitation of p75NTR in the four groups. β-Actin was used as a loading control. Data are shown as mean ± S.D. of three separate experiments performed in triplicate. The data were compared with the independent samples using One-way ANOVA.*p < 0.05.