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. 2019 Jun 5;10:1251. doi: 10.3389/fmicb.2019.01251

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Copyright © 2019 Vázquez and García.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Synergistic bactericidal activity of Cpl-711/PL3 on S. pneumoniae P042 planktonic cells and biofilms. (A) Viable cells of planktonic (nonadherent) fraction of the cultures. (B) Viable cells of biofilms. Treatments with each enzyme, alone or in combination, were at the indicated concentrations (in times the MIC), according to the protocol explained in Materials and Methods. First bar represents the sum of the independent effects of adding either 0.25× MIC of Cpl-711 or 0.25× MIC of PL3, while second bar shows the effect of adding 0.25× MIC of Cpl-711 and 0.25× MIC of PL3 at the same time. Results are the mean ± SD of viable count differences with respect to the controls in log scale for at least three independent experiments. Log kills marked with the same letter are not significantly different from one another, whereas those with different letters are significantly different from one another, according to ANOVA followed with Tukey post-test (P < 0.05).