Table 2.
MICs (μg/ml) for each lysin used in this work determined for several S. pneumoniae strainsa.
| ATCC 49619 | P042 | R6 | D39 | 48 | 69 | |
|---|---|---|---|---|---|---|
| Cpl-7 | >100 | 19.8 | 21.0 | 19.8 | 19.8 | >100 |
| Cpl-1 | 17.7 | 4.0 | 4.3 | 3.1 | 4.5 | 8.2 |
| Pal | >100 | 3.1 | 9.9 | 3.9 | 63.0 | 50 |
| LytA | 0.6 | 0.3 | 0.4 | 0.2 | 0.4 | 0.4 |
| Cpl-711 | 4.6 | 1.0 | 1.3 | 0.8 | 1.3 | 4.0 |
| PL3 | 0.5 | 0.2 | 0.1 | 0.2 | 0.3 | 0.2 |
| Cpl-1 vs. Cpl-711b | *** | *** | *** | *** | *** | ns |
| LytA vs. PL3b | ns | * | ** | ns | ns | ns |
a
Data are the geometric mean of at least three independent MIC experiments.
b
Statistical significance of comparisons between MICs of chimeric lysins and their most potent parental enzyme according to two-way ANOVA followed by Bonferroni post-test performed on the log2-transformed MIC values is shown below the table (*P < 0.05; **P < 0.01; ***P < 0.001; ns = non-significant difference).