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. 2019 Jun 5;10:1218. doi: 10.3389/fmicb.2019.01218

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Copyright © 2019 Berni, Soscia, Djermoun, Ize and Bleves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tla3 interaction with Tle3 (A) and VgrG2b (B–D). Copurification assay on StrepTactin column of Tla3^c_STREP with Tle3^c_His produced in E. coli BL21(DE3)pLysS from pBB27 and pVT1, respectively, (A) or of Tla3^c_His with VgrG2b^FL_STREP (B) or VgrG2b^FLD3_STREP (C) _or VgrG2b^FLD2-3_STREP (D) produced from pBB28 and pBB25 (B) or pBB41(C) or pBB42 (D). The unbound (U) and eluted (E) fractions were collected and subjected to SDS-PAGE (10.5%) and Western blot analysis using anti-His antibody (upper) and anti-streptavidin antibody (lower). The dashed line separates lanes from non-adjacent part of the same gel. The position of the proteins and the molecular mass markers (in kDa) are indicated.