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. 2019 Jun 5;10:1262. doi: 10.3389/fimmu.2019.01262

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Copyright © 2019 Levy, Reger, Segerberg, Lambert, Leijonhufvud, Baumer, Carlsten and Childs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Commonly used methods to manipulate and prepare NK cells for adoptive infusion decrease surface expression of CXCR4 and reduce their ability to migrate toward SDF-1α in vitro. Surface expression of CXCR4 was evaluated by flow cytometry. (A) CXCR4 surface expression on fresh T cells, B cells and NK cells among PBMC. Representative histograms are shown (one representative donor). (B) CXCR4 intensity, as displayed as GMFI relative to FMO, on different NK cell preparations (n = 10 donors). (C) In vitro transwell migration of NK cells from populations in (B) toward SDF-1α (n = 10 donors). Statistical significance was evaluated with the paired t-test, two-tailed. Bar graphs present the mean and error bars report the SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001.