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. 2019 Jun 5;10:1262. doi: 10.3389/fimmu.2019.01262

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Copyright © 2019 Levy, Reger, Segerberg, Lambert, Leijonhufvud, Baumer, Carlsten and Childs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ex vivo expanded NK cells transfected with CXCR4^R334X-coding mRNA have superior trafficking to bone marrow compartments 24 h following adoptive transfer compared to their mock transfected counterpart. (A) Experimental schematic illustrating in vivo trafficking of infused NK cells transfected with mRNA coding for luciferase and CXCR4^R334X or luciferase alone. (B) X-ray image of a mouse demonstrating ventral (top) and dorsal (bottom) anatomical views of NK cell distributions observed in (C). (C) Ventral (top) and dorsal (bottom) bioluminescence images of luciferase expressing NK cells 2 and 24 h after infusion into mice (n = 3 mice/group).